1 00:00:39,490 --> 00:00:55,450 go ahead Columbia copy that Mike stand 2 00:00:55,450 --> 00:00:55,460 go ahead Columbia copy that Mike stand 3 00:00:55,460 --> 00:01:07,240 go ahead Columbia copy that Mike stand by one for any instructions Columbia 4 00:01:07,240 --> 00:01:07,250 by one for any instructions Columbia 5 00:01:07,250 --> 00:01:15,850 by one for any instructions Columbia hunt oh yeah my god they're ready to let 6 00:01:15,850 --> 00:01:15,860 hunt oh yeah my god they're ready to let 7 00:01:15,860 --> 00:01:17,140 hunt oh yeah my god they're ready to let you go ahead and activate those to 8 00:01:17,140 --> 00:01:17,150 you go ahead and activate those to 9 00:01:17,150 --> 00:01:25,719 you go ahead and activate those to cancel also in addition to continuing 10 00:01:25,719 --> 00:01:25,729 cancel also in addition to continuing 11 00:01:25,729 --> 00:01:27,700 cancel also in addition to continuing work on the mid-deck of Columbia 12 00:01:27,700 --> 00:01:27,710 work on the mid-deck of Columbia 13 00:01:27,710 --> 00:01:29,740 work on the mid-deck of Columbia mission specialist Mike Lopez Alegria 14 00:01:29,740 --> 00:01:29,750 mission specialist Mike Lopez Alegria 15 00:01:29,750 --> 00:01:32,020 mission specialist Mike Lopez Alegria will also take some time to exercise 16 00:01:32,020 --> 00:01:32,030 will also take some time to exercise 17 00:01:32,030 --> 00:01:34,120 will also take some time to exercise today on the bicycle ergometer that will 18 00:01:34,120 --> 00:01:34,130 today on the bicycle ergometer that will 19 00:01:34,130 --> 00:01:37,480 today on the bicycle ergometer that will be set up on Columbia's mid deck Mike 20 00:01:37,480 --> 00:01:37,490 be set up on Columbia's mid deck Mike 21 00:01:37,490 --> 00:01:39,550 be set up on Columbia's mid deck Mike Lopez Alegria is also the astronaut on a 22 00:01:39,550 --> 00:01:39,560 Lopez Alegria is also the astronaut on a 23 00:01:39,560 --> 00:01:42,490 Lopez Alegria is also the astronaut on a blue team can you verify that that is 24 00:01:42,490 --> 00:01:42,500 blue team can you verify that that is 25 00:01:42,500 --> 00:01:58,109 blue team can you verify that that is Romeo tan echo that you're looking in 26 00:01:58,109 --> 00:01:58,119 27 00:01:58,119 --> 00:02:01,920 copy that Brett the Zaca CEQA insertion 28 00:02:01,920 --> 00:02:01,930 copy that Brett the Zaca CEQA insertion 29 00:02:01,930 --> 00:02:08,910 copy that Brett the Zaca CEQA insertion is complete that's affirmative son now 30 00:02:08,910 --> 00:02:08,920 is complete that's affirmative son now 31 00:02:08,920 --> 00:02:15,259 is complete that's affirmative son now step five point one four 32 00:02:15,259 --> 00:02:15,269 33 00:02:15,269 --> 00:02:31,899 copy that Fred five point one 34 00:02:31,899 --> 00:02:31,909 35 00:02:31,909 --> 00:02:34,190 they sell phones for Katy we did not 36 00:02:34,190 --> 00:02:34,200 they sell phones for Katy we did not 37 00:02:34,200 --> 00:03:12,839 they sell phones for Katy we did not hear that affirmative 38 00:03:12,839 --> 00:03:12,849 39 00:03:12,849 --> 00:03:16,240 and Katie that is the termination fluid 40 00:03:16,240 --> 00:03:16,250 and Katie that is the termination fluid 41 00:03:16,250 --> 00:03:18,699 and Katie that is the termination fluid the top plunger and the center plunger 42 00:03:18,699 --> 00:03:18,709 the top plunger and the center plunger 43 00:03:18,709 --> 00:03:20,229 the top plunger and the center plunger would terminate so we do not want to 44 00:03:20,229 --> 00:03:20,239 would terminate so we do not want to 45 00:03:20,239 --> 00:04:03,100 would terminate so we do not want to close that small gap 46 00:04:03,100 --> 00:04:03,110 47 00:04:03,110 --> 00:04:12,700 I'm so sad for a CBC go ahead put them 48 00:04:12,700 --> 00:04:12,710 I'm so sad for a CBC go ahead put them 49 00:04:12,710 --> 00:04:16,360 I'm so sad for a CBC go ahead put them under step one point one six assists to 50 00:04:16,360 --> 00:04:16,370 under step one point one six assists to 51 00:04:16,370 --> 00:04:18,759 under step one point one six assists to remove kept on tape from the isolator 52 00:04:18,759 --> 00:04:18,769 remove kept on tape from the isolator 53 00:04:18,769 --> 00:04:24,510 remove kept on tape from the isolator from both hands let me sit on one hand 54 00:04:24,510 --> 00:04:24,520 55 00:04:24,520 --> 00:07:07,180 copy different 56 00:07:07,180 --> 00:07:07,190 57 00:07:07,190 --> 00:07:10,790 also upcoming for the crew in about 20 58 00:07:10,790 --> 00:07:10,800 also upcoming for the crew in about 20 59 00:07:10,800 --> 00:07:12,649 also upcoming for the crew in about 20 minutes one of the final activities for 60 00:07:12,649 --> 00:07:12,659 minutes one of the final activities for 61 00:07:12,659 --> 00:07:14,180 minutes one of the final activities for the blue team will be a test of a new 62 00:07:14,180 --> 00:07:14,190 the blue team will be a test of a new 63 00:07:14,190 --> 00:07:16,670 the blue team will be a test of a new system being flown for the first time on 64 00:07:16,670 --> 00:07:16,680 system being flown for the first time on 65 00:07:16,680 --> 00:07:19,159 system being flown for the first time on a shuttle onboard duct Columbian STS 73 66 00:07:19,159 --> 00:07:19,169 a shuttle onboard duct Columbian STS 73 67 00:07:19,169 --> 00:07:21,350 a shuttle onboard duct Columbian STS 73 that system a system that allows a 68 00:07:21,350 --> 00:07:21,360 that system a system that allows a 69 00:07:21,360 --> 00:07:23,570 that system a system that allows a ground tear television to be sent to 70 00:07:23,570 --> 00:07:23,580 ground tear television to be sent to 71 00:07:23,580 --> 00:07:25,430 ground tear television to be sent to television from the ground to be sent up 72 00:07:25,430 --> 00:07:25,440 television from the ground to be sent up 73 00:07:25,440 --> 00:07:28,070 television from the ground to be sent up a to the crew and a vice versa 74 00:07:28,070 --> 00:07:28,080 a to the crew and a vice versa 75 00:07:28,080 --> 00:07:29,659 a to the crew and a vice versa two-way television essentially after 76 00:07:29,659 --> 00:07:29,669 two-way television essentially after 77 00:07:29,669 --> 00:07:32,210 two-way television essentially after two-way conversations again the first 78 00:07:32,210 --> 00:07:32,220 two-way conversations again the first 79 00:07:32,220 --> 00:07:34,219 two-way conversations again the first test of that system which is being flown 80 00:07:34,219 --> 00:07:34,229 test of that system which is being flown 81 00:07:34,229 --> 00:07:36,529 test of that system which is being flown is a test objective on this flight will 82 00:07:36,529 --> 00:07:36,539 is a test objective on this flight will 83 00:07:36,539 --> 00:07:39,110 is a test objective on this flight will be taking place in just about 20 minutes 84 00:07:39,110 --> 00:07:39,120 be taking place in just about 20 minutes 85 00:07:39,120 --> 00:07:50,600 be taking place in just about 20 minutes or so this concludes the pre-recorded 86 00:07:50,600 --> 00:07:50,610 or so this concludes the pre-recorded 87 00:07:50,610 --> 00:07:57,320 or so this concludes the pre-recorded oblique transmission of the GA TV this 88 00:07:57,320 --> 00:07:57,330 oblique transmission of the GA TV this 89 00:07:57,330 --> 00:07:59,029 oblique transmission of the GA TV this is Mission Control Houston now that 90 00:07:59,029 --> 00:07:59,039 is Mission Control Houston now that 91 00:07:59,039 --> 00:08:01,640 is Mission Control Houston now that again has been a test of ground to air 92 00:08:01,640 --> 00:08:01,650 again has been a test of ground to air 93 00:08:01,650 --> 00:08:04,939 again has been a test of ground to air television uplink to Columbia that 94 00:08:04,939 --> 00:08:04,949 television uplink to Columbia that 95 00:08:04,949 --> 00:08:07,040 television uplink to Columbia that equipment being flown for the first time 96 00:08:07,040 --> 00:08:07,050 equipment being flown for the first time 97 00:08:07,050 --> 00:08:11,839 equipment being flown for the first time on this flight again allowing scenes to 98 00:08:11,839 --> 00:08:11,849 on this flight again allowing scenes to 99 00:08:11,849 --> 00:08:15,110 on this flight again allowing scenes to be sent up to the shuttle from the 100 00:08:15,110 --> 00:08:15,120 be sent up to the shuttle from the 101 00:08:15,120 --> 00:08:17,360 be sent up to the shuttle from the ground from Mission Control that segment 102 00:08:17,360 --> 00:08:17,370 ground from Mission Control that segment 103 00:08:17,370 --> 00:08:18,950 ground from Mission Control that segment just a test segment with several 104 00:08:18,950 --> 00:08:18,960 just a test segment with several 105 00:08:18,960 --> 00:08:20,719 just a test segment with several different test patterns and several 106 00:08:20,719 --> 00:08:20,729 different test patterns and several 107 00:08:20,729 --> 00:08:22,279 different test patterns and several different highlights from past shuttle 108 00:08:22,279 --> 00:08:22,289 different highlights from past shuttle 109 00:08:22,289 --> 00:08:28,959 different highlights from past shuttle flights just methods of illustrating the 110 00:08:28,959 --> 00:08:28,969 111 00:08:28,969 --> 00:08:32,269 this shot now is live from Mission 112 00:08:32,269 --> 00:08:32,279 this shot now is live from Mission 113 00:08:32,279 --> 00:08:33,949 this shot now is live from Mission Control of a flight director Brian 114 00:08:33,949 --> 00:08:33,959 Control of a flight director Brian 115 00:08:33,959 --> 00:08:36,529 Control of a flight director Brian Austin and spacecraft communicator Carl 116 00:08:36,529 --> 00:08:36,539 Austin and spacecraft communicator Carl 117 00:08:36,539 --> 00:08:41,930 Austin and spacecraft communicator Carl Meade again being sent up to Columbia as 118 00:08:41,930 --> 00:08:41,940 Meade again being sent up to Columbia as 119 00:08:41,940 --> 00:08:44,630 Meade again being sent up to Columbia as well again Columbia the first shuttle to 120 00:08:44,630 --> 00:08:44,640 well again Columbia the first shuttle to 121 00:08:44,640 --> 00:08:46,490 well again Columbia the first shuttle to fly with this equipment it's a test 122 00:08:46,490 --> 00:08:46,500 fly with this equipment it's a test 123 00:08:46,500 --> 00:08:48,199 fly with this equipment it's a test objective several other tests of the 124 00:08:48,199 --> 00:08:48,209 objective several other tests of the 125 00:08:48,209 --> 00:08:50,319 objective several other tests of the ground air television capability will be 126 00:08:50,319 --> 00:08:50,329 ground air television capability will be 127 00:08:50,329 --> 00:08:53,300 ground air television capability will be performed throughout the 16 days a 128 00:08:53,300 --> 00:08:53,310 performed throughout the 16 days a 129 00:08:53,310 --> 00:08:58,819 performed throughout the 16 days a Columbia spins in general and we can see 130 00:08:58,819 --> 00:08:58,829 Columbia spins in general and we can see 131 00:08:58,829 --> 00:09:00,800 Columbia spins in general and we can see payload specialist al Sacco they're 132 00:09:00,800 --> 00:09:00,810 payload specialist al Sacco they're 133 00:09:00,810 --> 00:09:02,930 payload specialist al Sacco they're going into one of the stowage containers 134 00:09:02,930 --> 00:09:02,940 going into one of the stowage containers 135 00:09:02,940 --> 00:09:06,220 going into one of the stowage containers on the ceiling of the Space Lab module 136 00:09:06,220 --> 00:09:06,230 on the ceiling of the Space Lab module 137 00:09:06,230 --> 00:09:11,949 on the ceiling of the Space Lab module there are there's a great variety of 138 00:09:11,949 --> 00:09:11,959 139 00:09:11,959 --> 00:09:14,150 apparatus that has to be stored in 140 00:09:14,150 --> 00:09:14,160 apparatus that has to be stored in 141 00:09:14,160 --> 00:09:17,680 apparatus that has to be stored in special areas of the module or 142 00:09:17,680 --> 00:09:17,690 special areas of the module or 143 00:09:17,690 --> 00:09:23,380 special areas of the module or the shuttle mid-deck in lockers and all 144 00:09:23,380 --> 00:09:23,390 the shuttle mid-deck in lockers and all 145 00:09:23,390 --> 00:09:25,690 the shuttle mid-deck in lockers and all of that comes under the heading of 146 00:09:25,690 --> 00:09:25,700 of that comes under the heading of 147 00:09:25,700 --> 00:09:29,590 of that comes under the heading of stowage it's a one specialized aspect of 148 00:09:29,590 --> 00:09:29,600 stowage it's a one specialized aspect of 149 00:09:29,600 --> 00:09:31,770 stowage it's a one specialized aspect of preparing for a shuttle mission is just 150 00:09:31,770 --> 00:09:31,780 preparing for a shuttle mission is just 151 00:09:31,780 --> 00:09:33,820 preparing for a shuttle mission is just planning out where you're going to put 152 00:09:33,820 --> 00:09:33,830 planning out where you're going to put 153 00:09:33,830 --> 00:09:35,170 planning out where you're going to put all the things that the crew will need 154 00:09:35,170 --> 00:09:35,180 all the things that the crew will need 155 00:09:35,180 --> 00:09:40,800 all the things that the crew will need to get access to for launch and landing 156 00:09:40,800 --> 00:09:40,810 to get access to for launch and landing 157 00:09:40,810 --> 00:09:42,700 to get access to for launch and landing many of the things that they'll be using 158 00:09:42,700 --> 00:09:42,710 many of the things that they'll be using 159 00:09:42,710 --> 00:09:45,880 many of the things that they'll be using during the course of the mission are put 160 00:09:45,880 --> 00:09:45,890 during the course of the mission are put 161 00:09:45,890 --> 00:09:52,390 during the course of the mission are put away in particular areas so that they 162 00:09:52,390 --> 00:09:52,400 away in particular areas so that they 163 00:09:52,400 --> 00:09:54,460 away in particular areas so that they obviously saw that they aren't just 164 00:09:54,460 --> 00:09:54,470 obviously saw that they aren't just 165 00:09:54,470 --> 00:09:57,330 obviously saw that they aren't just floating around loose during those 166 00:09:57,330 --> 00:09:57,340 floating around loose during those 167 00:09:57,340 --> 00:10:02,890 floating around loose during those launch and landing periods and so now we 168 00:10:02,890 --> 00:10:02,900 launch and landing periods and so now we 169 00:10:02,900 --> 00:10:05,380 launch and landing periods and so now we get a better look with Sacco in the 170 00:10:05,380 --> 00:10:05,390 get a better look with Sacco in the 171 00:10:05,390 --> 00:10:07,770 get a better look with Sacco in the foreground pulling down one of those 172 00:10:07,770 --> 00:10:07,780 foreground pulling down one of those 173 00:10:07,780 --> 00:10:11,200 foreground pulling down one of those stowage areas it's like a drawer that in 174 00:10:11,200 --> 00:10:11,210 stowage areas it's like a drawer that in 175 00:10:11,210 --> 00:10:13,060 stowage areas it's like a drawer that in this case though comes down vertically 176 00:10:13,060 --> 00:10:13,070 this case though comes down vertically 177 00:10:13,070 --> 00:11:39,480 this case though comes down vertically from the ceiling of the Space Lab module 178 00:11:39,480 --> 00:11:39,490 179 00:11:39,490 --> 00:11:47,610 Space Lab Huntsville with words on DPM 180 00:11:47,610 --> 00:11:47,620 181 00:11:47,620 --> 00:11:50,830 yes Kathy DPM would like you to manually 182 00:11:50,830 --> 00:11:50,840 yes Kathy DPM would like you to manually 183 00:11:50,840 --> 00:11:54,580 yes Kathy DPM would like you to manually tune X to start with and see what 184 00:11:54,580 --> 00:11:54,590 tune X to start with and see what 185 00:11:54,590 --> 00:12:48,710 tune X to start with and see what happens with that 186 00:12:48,710 --> 00:12:48,720 187 00:12:48,720 --> 00:12:51,470 this is in the Astro culture plant 188 00:12:51,470 --> 00:12:51,480 this is in the Astro culture plant 189 00:12:51,480 --> 00:12:54,590 this is in the Astro culture plant growth experiment system and it's the 190 00:12:54,590 --> 00:12:54,600 growth experiment system and it's the 191 00:12:54,600 --> 00:12:56,869 growth experiment system and it's the first opportunity that the science team 192 00:12:56,869 --> 00:12:56,879 first opportunity that the science team 193 00:12:56,879 --> 00:12:59,509 first opportunity that the science team for that experiment is having to take a 194 00:12:59,509 --> 00:12:59,519 for that experiment is having to take a 195 00:12:59,519 --> 00:13:03,019 for that experiment is having to take a look at sources for the next several 196 00:13:03,019 --> 00:13:03,029 look at sources for the next several 197 00:13:03,029 --> 00:13:09,590 look at sources for the next several minutes this is their first opportunity 198 00:13:09,590 --> 00:13:09,600 minutes this is their first opportunity 199 00:13:09,600 --> 00:13:13,009 minutes this is their first opportunity to see get a little insight into the 200 00:13:13,009 --> 00:13:13,019 to see get a little insight into the 201 00:13:13,019 --> 00:13:16,749 to see get a little insight into the status of the plant growth inside that 202 00:13:16,749 --> 00:13:16,759 status of the plant growth inside that 203 00:13:16,759 --> 00:13:24,249 status of the plant growth inside that facility which is which is intended to 204 00:13:24,249 --> 00:13:24,259 205 00:13:24,259 --> 00:13:29,689 allow potato seedlings to grow in 206 00:13:29,689 --> 00:13:29,699 allow potato seedlings to grow in 207 00:13:29,699 --> 00:13:33,889 allow potato seedlings to grow in microgravity conditions the principal 208 00:13:33,889 --> 00:13:33,899 microgravity conditions the principal 209 00:13:33,899 --> 00:13:35,720 microgravity conditions the principal investigator team here in Huntsville 210 00:13:35,720 --> 00:13:35,730 investigator team here in Huntsville 211 00:13:35,730 --> 00:13:38,960 investigator team here in Huntsville advised that they're elated at the way 212 00:13:38,960 --> 00:13:38,970 advised that they're elated at the way 213 00:13:38,970 --> 00:13:43,910 advised that they're elated at the way things look inside the facility it's a 214 00:13:43,910 --> 00:13:43,920 things look inside the facility it's a 215 00:13:43,920 --> 00:13:48,230 things look inside the facility it's a rather close-up view showing the tops of 216 00:13:48,230 --> 00:13:48,240 rather close-up view showing the tops of 217 00:13:48,240 --> 00:13:52,220 rather close-up view showing the tops of the leaflets there are about three of 218 00:13:52,220 --> 00:13:52,230 the leaflets there are about three of 219 00:13:52,230 --> 00:13:55,759 the leaflets there are about three of the small potato plant leaflets that are 220 00:13:55,759 --> 00:13:55,769 the small potato plant leaflets that are 221 00:13:55,769 --> 00:13:58,699 the small potato plant leaflets that are visible in this very detailed view the 222 00:13:58,699 --> 00:13:58,709 visible in this very detailed view the 223 00:13:58,709 --> 00:14:04,519 visible in this very detailed view the tubers that are the actual substance of 224 00:14:04,519 --> 00:14:04,529 tubers that are the actual substance of 225 00:14:04,529 --> 00:14:10,689 tubers that are the actual substance of the potatoes yes source of starch are 226 00:14:10,689 --> 00:14:10,699 the potatoes yes source of starch are 227 00:14:10,699 --> 00:14:14,439 the potatoes yes source of starch are buried or obscured by the leaflets and 228 00:14:14,439 --> 00:14:14,449 buried or obscured by the leaflets and 229 00:14:14,449 --> 00:14:18,170 buried or obscured by the leaflets and but the science team members are able to 230 00:14:18,170 --> 00:14:18,180 but the science team members are able to 231 00:14:18,180 --> 00:14:21,499 but the science team members are able to judge the status of growth from what 232 00:14:21,499 --> 00:14:21,509 judge the status of growth from what 233 00:14:21,509 --> 00:14:24,079 judge the status of growth from what they see in this video and they've 234 00:14:24,079 --> 00:14:24,089 they see in this video and they've 235 00:14:24,089 --> 00:14:27,110 they see in this video and they've indicated that what they see tells them 236 00:14:27,110 --> 00:14:27,120 indicated that what they see tells them 237 00:14:27,120 --> 00:14:28,790 indicated that what they see tells them that the plants so far at least are 238 00:14:28,790 --> 00:14:28,800 that the plants so far at least are 239 00:14:28,800 --> 00:14:31,879 that the plants so far at least are maintaining the vigor that they would 240 00:14:31,879 --> 00:14:31,889 maintaining the vigor that they would 241 00:14:31,889 --> 00:14:41,269 maintaining the vigor that they would like to see 242 00:14:41,269 --> 00:14:41,279 243 00:14:41,279 --> 00:14:46,170 and of course all of this is being done 244 00:14:46,170 --> 00:14:46,180 and of course all of this is being done 245 00:14:46,180 --> 00:14:49,069 and of course all of this is being done as preliminaries preliminary steps to 246 00:14:49,069 --> 00:14:49,079 as preliminaries preliminary steps to 247 00:14:49,079 --> 00:14:51,720 as preliminaries preliminary steps to starting to go into the actual science 248 00:14:51,720 --> 00:14:51,730 starting to go into the actual science 249 00:14:51,730 --> 00:15:16,110 starting to go into the actual science work with liquid drops in the chamber 250 00:15:16,110 --> 00:15:16,120 251 00:15:16,120 --> 00:15:18,749 and Kathy we'd like you to go ahead and 252 00:15:18,749 --> 00:15:18,759 and Kathy we'd like you to go ahead and 253 00:15:18,759 --> 00:15:20,639 and Kathy we'd like you to go ahead and move the injectors in please fully 254 00:15:20,639 --> 00:15:20,649 move the injectors in please fully 255 00:15:20,649 --> 00:15:46,620 move the injectors in please fully inserted 256 00:15:46,620 --> 00:15:46,630 257 00:15:46,630 --> 00:15:49,819 and now we could see those injectors 258 00:15:49,819 --> 00:15:49,829 and now we could see those injectors 259 00:15:49,829 --> 00:15:55,319 and now we could see those injectors coming in and contacting the small solid 260 00:15:55,319 --> 00:15:55,329 coming in and contacting the small solid 261 00:15:55,329 --> 00:16:00,030 coming in and contacting the small solid sphere immobilizing the sphere this is 262 00:16:00,030 --> 00:16:00,040 sphere immobilizing the sphere this is 263 00:16:00,040 --> 00:16:13,079 sphere immobilizing the sphere this is another of the steps that's affirmative 264 00:16:13,079 --> 00:16:13,089 another of the steps that's affirmative 265 00:16:13,089 --> 00:16:29,160 another of the steps that's affirmative Cathy we'd like to see that 266 00:16:29,160 --> 00:16:29,170 267 00:16:29,170 --> 00:16:31,750 we get a pretty good close-up view of 268 00:16:31,750 --> 00:16:31,760 we get a pretty good close-up view of 269 00:16:31,760 --> 00:16:34,770 we get a pretty good close-up view of the liquid injector systems here in this 270 00:16:34,770 --> 00:16:34,780 the liquid injector systems here in this 271 00:16:34,780 --> 00:16:38,200 the liquid injector systems here in this video that's coming down they are one of 272 00:16:38,200 --> 00:16:38,210 video that's coming down they are one of 273 00:16:38,210 --> 00:16:40,300 video that's coming down they are one of the portions of this system that have 274 00:16:40,300 --> 00:16:40,310 the portions of this system that have 275 00:16:40,310 --> 00:16:42,970 the portions of this system that have been improved since the first flight on 276 00:16:42,970 --> 00:16:42,980 been improved since the first flight on 277 00:16:42,980 --> 00:16:49,210 been improved since the first flight on STS 50 us ml one the injectors and tips 278 00:16:49,210 --> 00:16:49,220 STS 50 us ml one the injectors and tips 279 00:16:49,220 --> 00:16:55,660 STS 50 us ml one the injectors and tips have been redesigned hand over to 280 00:16:55,660 --> 00:16:55,670 have been redesigned hand over to 281 00:16:55,670 --> 00:17:04,930 have been redesigned hand over to teacher sees this is space will have 282 00:17:04,930 --> 00:17:04,940 teacher sees this is space will have 283 00:17:04,940 --> 00:17:06,970 teacher sees this is space will have operations Huntsville where we are 284 00:17:06,970 --> 00:17:06,980 operations Huntsville where we are 285 00:17:06,980 --> 00:17:09,190 operations Huntsville where we are currently receiving downlink video from 286 00:17:09,190 --> 00:17:09,200 currently receiving downlink video from 287 00:17:09,200 --> 00:17:13,660 currently receiving downlink video from inside the chamber of the single Locker 288 00:17:13,660 --> 00:17:13,670 inside the chamber of the single Locker 289 00:17:13,670 --> 00:17:16,630 inside the chamber of the single Locker protein crystal growth experiment this 290 00:17:16,630 --> 00:17:16,640 protein crystal growth experiment this 291 00:17:16,640 --> 00:17:19,060 protein crystal growth experiment this particular experiment is crystal growth 292 00:17:19,060 --> 00:17:19,070 particular experiment is crystal growth 293 00:17:19,070 --> 00:17:22,330 particular experiment is crystal growth by liquid liquid diffusion for this 294 00:17:22,330 --> 00:17:22,340 by liquid liquid diffusion for this 295 00:17:22,340 --> 00:17:24,310 by liquid liquid diffusion for this experiment the protein crystals are 296 00:17:24,310 --> 00:17:24,320 experiment the protein crystals are 297 00:17:24,320 --> 00:17:27,190 experiment the protein crystals are grown in for handheld diffusion test 298 00:17:27,190 --> 00:17:27,200 grown in for handheld diffusion test 299 00:17:27,200 --> 00:17:30,150 grown in for handheld diffusion test cells which are often referred to as a 300 00:17:30,150 --> 00:17:30,160 cells which are often referred to as a 301 00:17:30,160 --> 00:17:34,600 cells which are often referred to as a THD t sees each one of these contains 302 00:17:34,600 --> 00:17:34,610 THD t sees each one of these contains 303 00:17:34,610 --> 00:17:39,070 THD t sees each one of these contains four test cells and by diffusing one 304 00:17:39,070 --> 00:17:39,080 four test cells and by diffusing one 305 00:17:39,080 --> 00:17:42,370 four test cells and by diffusing one liquid into another crystal growth is 306 00:17:42,370 --> 00:17:42,380 liquid into another crystal growth is 307 00:17:42,380 --> 00:17:46,030 liquid into another crystal growth is accomplished in this particular 308 00:17:46,030 --> 00:17:46,040 accomplished in this particular 309 00:17:46,040 --> 00:17:49,060 accomplished in this particular experiment the two different liquids are 310 00:17:49,060 --> 00:17:49,070 experiment the two different liquids are 311 00:17:49,070 --> 00:17:51,250 experiment the two different liquids are brought into contact with each other but 312 00:17:51,250 --> 00:17:51,260 brought into contact with each other but 313 00:17:51,260 --> 00:17:54,340 brought into contact with each other but they are not actually mixed eventually 314 00:17:54,340 --> 00:17:54,350 they are not actually mixed eventually 315 00:17:54,350 --> 00:17:57,670 they are not actually mixed eventually these fluids will intermingle with one 316 00:17:57,670 --> 00:17:57,680 these fluids will intermingle with one 317 00:17:57,680 --> 00:18:00,040 these fluids will intermingle with one another what is often refer to as 318 00:18:00,040 --> 00:18:00,050 another what is often refer to as 319 00:18:00,050 --> 00:18:03,190 another what is often refer to as diffusion through the random motion of 320 00:18:03,190 --> 00:18:03,200 diffusion through the random motion of 321 00:18:03,200 --> 00:18:06,250 diffusion through the random motion of molecules by gradually increasing the 322 00:18:06,250 --> 00:18:06,260 molecules by gradually increasing the 323 00:18:06,260 --> 00:18:08,650 molecules by gradually increasing the concentration of the precipitant in the 324 00:18:08,650 --> 00:18:08,660 concentration of the precipitant in the 325 00:18:08,660 --> 00:18:12,310 concentration of the precipitant in the protein solution this causes nucleation 326 00:18:12,310 --> 00:18:12,320 protein solution this causes nucleation 327 00:18:12,320 --> 00:18:22,549 protein solution this causes nucleation of the crystals 328 00:18:22,549 --> 00:18:22,559 329 00:18:22,559 --> 00:18:24,620 you